2013-2014 CIF COURSES
" Introduction to fluorescence imaging for the analysis of living cells "
Cours de privat docent et Cellular Imaging Facility (CIF)
Donné par Dr Jean-Yves CHATTON
Place : Petit Auditoire de l'Ecole de Médecine, rue du Bugnon 9 (1er étage)
Schedule : Winter Semester – Tuesdays 12h15 - 14h00
07 January 2014: Basics of transmitted light and fluorescence microscopy.
14 January 2014: Confocal microscopy.
21 January 2014: Modes of image formation, acquisition, signal sampling.
28 January 2014: Dynamic recording of cellular functions by fluorescence imaging.
Intracellular calcium, pH, and sodium and their application to cellular signaling.
Problems related to imaging of living cells.
4 February 2013: Other optical applications (proposed topics):
Fluorescence recovery after photobleaching (FRAP), flash photolysis,multiphoton microscopy, evanescent wave microscopy, laser tweezers, fluorescence resonance energy transfer (FRET), digital holography microscopy, optical contrasting methods (phase contrast, DIC).
§ Lectures will be given in English
§ In parallel with theoretical lectures, demonstrations will be proposed on the confocal microscopes of the Cellular Imaging Facility (details and registration on site).
§ gives right to ONE credit for students of doctoral schools (FBM and neuroscience)
==> Admission to the course is free and open to anyone interested
(please register to
-- on site registration possible)