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How-to: Fast sequential acquisition for the Zeiss LSM 710 (Bugnon) - Zen 2009 PDF
Written by Yannick KREMPP   
Thursday, 26 March 2015

Upon request, here is a tutorial on how to set up the channels manually on the Zeiss LSM 710, to be able to do fast sequential acquisitions.

In a nutshell, you have to set up the channel configuration so that you have the same mirrors in every configuration, and switch between lines instead of between frames. 

One major advantage for you is a dramatic improvement of the acquisition time (and you could even go further by enabling bi-directional scanning...).

One major advantage for the setup is premature wear prevention for the filter wheel

 faster-710.png

The PDF version of the How-To is just here:

LSM 710 faster sequential acquisition How-To (PDF)

 

The CIF Staff 

Last Updated ( Thursday, 26 March 2015 )
 
LSM 780 (Bugnon) up and running PDF
Written by Yannick KREMPP   
Wednesday, 18 March 2015

After a few issues, the LSM 780 is back up and running.

You can book it as usual.

 Best regards,

 

The CIF Staff 

 
The new Zeiss LSM 780 confocal microscope at the Bugnon campus PDF
Written by Yannick KREMPP   
Tuesday, 17 February 2015
The new confocal microscope that replaces the old Leica SP5 is now up and running, and available for bookings and trainings.
 
For the CIF users already trained on the 710, the switch to the 780 should be relatively straightforward, and only a few minutes should be necessary to get the sense of what is different. New users will be trained on the 780 unless there’s a specific demand.
 
One major change from the 710 is the use of GaAsp detectors instead of the usual PMTs to detect the fluorescence signal. These detectors are much more sensitive, and are best suited for difficult samples and weak signal.
 
The Zen software is in its 2012 version, and comes with tweaks here and there in the user interface, but also with a much more comprehensive (and smart…) - Smart Setup.
lsm-780.png
If you need a training or a short introduction, please contact Yannick KREMPP before booking it for the first time.

The LSM 780 specs sheet
Inverted microscope with fully motorized stand, motorized stage, DIC and Phase, 32 GaAsp detectors, Quasar array for spectral detection and unmixing (34 channels), FCS, Transmission PMT.
 
Illumination
X-Cite lamp
 
Laser illumination
Diode 405 nm, Argon 458-476-488-514 nm, DPSS 561 nm, He/Ne 633 nm.
 
Objectives
- Plan Neofluar 10x / 0.30 NA 
- Plan Apochromat 20x / 0.8 NA
- Plan Neofluar 40x /0.6 NA 
- Plan Apochromat 40x / 1.30 NA / oil immersion
- Plan Apochromat 63x /1.40 NA / oil immersion
 
Software
Zen 2012

Last Updated ( Tuesday, 17 February 2015 )
 
New File Server at the CIF PDF
Written by Yannick KREMPP   
Friday, 13 February 2015

Dear CIF users,

We are currently in the process of migrating all our users to a new file server. This new server, ATHENA, based on Synology technology is more modern, robust, and has many more other advantages over the current system we use, ATLAS. The migration process will take time (several months at best), so you will be informed via email when your account has been processed.

atlas-to-athena-corrected.png

The new server has a data cap of 15 GB per user, so those who already have more than that won’t be able to add data to their home folder on ATHENA until they make room for it.

Special accounts will be treated separately, on a case-by- case basis.

Of course, if you encounter issues of any kind, please refer to Yannick KREMPP at This e-mail address is being protected from spam bots, you need JavaScript enabled to view it .

The CIF Staff.

Last Updated ( Friday, 13 February 2015 )
 
CIF SEMINAR PDF
Written by Yannick KREMPP   
Tuesday, 27 January 2015

CIF SEMINAR


Sample clearing technologies and deep tissue imaging with the UltraMicroscope 2 light sheet microscope :
Whole organ imaging with cellular resolution
 
by
Dr. Christian Feuillet
LaVision Biotec application scientist, Bielefeld, Germany


Tuesday February 10, 2015 - 12:15 PM
Room B301 (Epalinges UNIL Site, Building B)



The seminar by Dr. Christian Feuillet will cover major approaches of tissue clearing and imaging of large transparent tissue samples using light sheet microscopy. This type of technology has recently been installed at CIF Epalinges and is accessible to CIF users.


For information on the seminar or on the Ultramicroscope light sheet microscope at CIF, please contact Florence Morgenthaler, CIF Epalinges Technical Manager ( This e-mail address is being protected from spam bots, you need JavaScript enabled to view it ; tel. 021-692-5891).
 
*** UPDATE ***
Room changed from B305 to B301
 
Last Updated ( Wednesday, 28 January 2015 )
 
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