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ZEISS LSM 710 out of order at the Bugnon campus PDF
Written by Yannick KREMPP   
Friday, 17 October 2014

Dear CIF Users,  the ZEISS LSM 710 confocal at the Bugnon campus is out of order until further notice.

The faulty piece of hardware has been identified, and a replacement part has been ordered. The repair is scheduled for this end of next week at best.

If you have urgent needs of confocal microscopy at the Bugnon, we still have the Leica SP5 running, but know it will soon be dismantled and replaced by another Zeiss confocal. Another option is to check if the offer at the Dorigny or Epalinges campus could suit your needs until the repair has been done. 

We apologize for the inconvenience.

The CIF Staff 

 

 
Privat Docent course on imaging 2015 PDF
Written by Yannick KREMPP   
Tuesday, 26 August 2014
Cours de privat docent & Cellular Imaging Facility (CIF) 
Dr  Jean-Yves CHATTON 
 
 Introduction to fluorescence imaging for the analysis of living cells
 
Place: Petit Auditoire de l'Ecole de Médecine, rue du Bugnon 9 (1er étage)
Schedule: Winter Semester – Tuesdays    12:15 – 14:00
________________________________
 
6 January 2015 : Basics of transmitted light and fluorescence microscopy
13 January 2015 : Confocal microscopy 
20 January 2015 : Modes of image formation, acquisition, signal sampling
27 January 2015 : Dynamic recording of cellular functions by fluorescence imaging.
Intracellular ion imaging and cellular signaling.
Problems related to imaging of living cells
3 February 2015 : Other optical applications (proposed topics):
Fluorescence recovery after photobleaching (FRAP), UV flash photolysis, multiphoton microscopy, evanescent wave microscopy, fluorescence resonance energy transfer (FRET), optical contrasting methods (phase contrast, DIC), super- resolution microscopy

Remarks:
 
- lectures will be given in English.
- in parallel with theoretical lectures, demonstrations will be proposed on the confocal microscopes of the Cellular Imaging Facility (details and registration on site).
- gives right to ONE credit for students of doctoral schools (FBM and neuroscience)
 
Admission to the course is free and open to anyone interested
(Please register  to This e-mail address is being protected from spam bots, you need JavaScript enabled to view it  -- on site registration possible)

 
Leica SP5 at the Bugnon campus out of order *UPDATED* PDF
Written by Yannick KREMPP   
Thursday, 31 July 2014

Dear CIF Users,

The Leica SP5 confocal at the Bugnon campus is out of order until further notice. We apologize for the inconvenience and will keep you informed of any update on the situation.

The CIF Staff 

 *** UPDATE 06.08.2014 *** 

 

The Leica SP5 is back online after a couple of days of thorough testing. A few considerations should still catch your attention:

  • This system is becoming quite old now, and might not provide the best results you could achieve with more recent confocals.
  • Before operating the microscope, it needs at least half an hour with the lasers on to stabilize itself.
  • Some instabilities issues might remain, and in case of such bugs, just try to restart the whole setup to see if it fixes itself. In case it does not recover, please call someone from the CIF staff.
  • Finally, this system is due to replacement before the end of the year. People looking for a better Leica confocal experience can get in touch with the Epalinges and Dorigny platforms where Leica confocals are also installed.


 

Last Updated ( Wednesday, 06 August 2014 )
 
New Workstations at the CIF Bugnon PDF
Written by Yannick KREMPP   
Friday, 20 June 2014

achilleus-and-herakles.png

 

It was about time, but finally here they are: ACHILLEUS and HERAKLES (we like fancy names) will support the KRAKEN (told you) in the heavy duty image processing department at the Bugnon campus.

Each of these workstations has been equipped with 32 Gb of RAM, an Intel 3930K hexacore chip, 256 of fast SSD and 3Tb for the OS and working storage, and a 4 Gb Radeon R9 290x GPU able to chew up any kind of data set you throw at it in a breeze.

They both run Windows 7 Pro x64 (yep, not 8, we're this good with you), Office 2013 (maybe not that good after all, but we don't have a choice here) and of course: Imaris, Photoshop, Image J (in its Fiji flavour), Axiovision, Zen Blue and Black, and LAS AF. All these softwares are installed in their latest version.  

Fon non tech-savvy people this translates into a much more simple:

Use the KRAKEN for deconvolution and other heavy processor demanding software.
Use one of the greek demi-gods for Imaris or any other program relying on the GPU.

In any case, as these machines are brand new you might stumble on issues like missing programs, links or such. If it's the case, don't hesitate to ask Yannick Krempp at  This e-mail address is being protected from spam bots, you need JavaScript enabled to view it  or 5290 for support. (Oh, and please just avoid installing any funky software by your own means, it always ends up badly).

The CIF Staff 

Last Updated ( Friday, 20 June 2014 )
 
CIF Workshop 2014 - Colocalization - PDF Version available PDF
Written by Yannick KREMPP   
Wednesday, 18 June 2014
article-workshop-coloc-2014-pdf.png
 
The PDF version of the workshop is available using the following links:
 Part 1
 
The CIF Staff 
 
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