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Bugnon Resources 2016 Print E-mail
Written by Yannick KREMPP   
Tuesday, 10 May 2016

BUGNON

 

Acquisition Systems

Workstations

Zeiss Axioscan Z1

KRAKEN-B

Zeiss LSM 710 Quasar Confocal Microscope

ACHILLEUS

Zeiss LSM 780 Quasar Confocal Microscope

HERAKLES

Zeiss Time-Lapse setup

 

Zeiss Axiovision

 

Nikon Stereomicroscope SMZ 25

 

Stereomicroscope Leica MZ16FA

 

 

ACQUISITION SYSTEMS

 

Zeiss Axioscan Z1

 

SYSTEM SPECIFICATIONS:

·       fully automated for high throughput

·       Z-drive with 3mm usable focus range

·       Magazine for 100 slides 26x76mm

·       Precision scanning stage 300x100mm

·       light source for bright field: Vis-LED

·       light source for fluorescence: HXP 120 V (D)

·       for fixed samples

·       Software: Zen 2

 

2 CAMERAS:

Model: 3CCD Hitachi HV-F202SCL for Axio Scan.Z1 (O)

·       Type: High-End 3CCD progressive scan color camera.

·       Effective number of pixels: 1600 x 1200

·       Cell size: 4.4 µm x 4.4 µm

·       Effective Area: 7.04 mm x 5.28 mm

·       Max frame rate: 30 frames/s RGB

·       Dynamic range: 12 bit

·       Sensitivity: 2000 Lux/F5.6

 

 Model: Orca Flash 4.0 V2 for Axio Scan.Z1 (O)

·       Type: High-End monochrome with digital interface

·       Effective number of pixels: 2048 x 2048

·       Cell size: 6.5 µm x 6.5 µm

·       Effective Area: 13.31 mm x 13.31 mm

·       Spectral range: appr. 250 - 1000 nm

·       Camera Link frame grabber

·       full frame rate: 100 fps, at 20 ms,

·       Dynamic range: 16 bit

·       Quantum efficiency (at 600 nm): > 70%

·       noise at min EM gain: 1.6 electrons rms

 

OBJECTIVES

·       5x /0.25

·       10x /0.45

·       20x /0.80

·       40x /0.95

 

FLUOROCHROMES

FILTERSETS

Fluorochromes

Excitation

Dichroic

Emission

Filter set

DAPI

387/11

409

447/602

F36-513

eGFP

470/40

495

520 / 50

38 HE eGFP shift free (E)

Cy3

550/25

570

605/70

43 HE Cy 3 shift free (E)

mPlum

587/25

605

647/70

64 HE mPlum shift free (E)

Cy5

640/30

660

690/50

50 Cy 5 shift free (E)

 

Zeiss LSM 710 Quasar Confocal Microscope

 

Illumination

X-Cite lamp

 

Laser illumination

Diode 405 nm, Argon 458-476-488-514 nm, DPSS 561 nm, He/Ne 633 nm.

 

Objectives

·       Plan neofluar 10x / 0.30 NA (No DIC)

·       Plan neofluar 20x / 0.50 NA

·       Plan neofluar 40x dry

·       Plan neofluor 25x oil immersion

·       Plan neofluar 40x / 1.30 NA / oil immersion

·       Plan apochromat 63x /1.20 NA / oil immersion

·       Plan neofluar 100x /1.30 NA / oil immersion

Equipped with DIC, emission spectra imaging with the Quasar Detector.

 

Attached software

Zen 2012

 

Zeiss LSM 780 Quasar Confocal Microscope

 

Inverted microscope with fully motorized stand, motorized stage, DIC and Phase, 32 GaAsp detectors, Quasar array for spectral detection and unmixing (34 channels), FCS, Transmission PMT.

 

Illumination

X-Cite lamp

 

Laser illumination

Diode 405 nm, Argon 458-476-488-514 nm, DPSS 561 nm, He/Ne 633 nm.

 

Objectives

·       Plan Neofluar 10x / 0.30 NA

·       Plan Apochromat 20x / 0.8 NA

·       Plan Neofluar 40x /0.6 NA

·       Plan Apochromat 40x / 1.30 NA / oil immersion

·       Plan Apochromat 63x /1.40 NA / oil immersion

 

Software

Zen 2012

 

Zeiss Time-Lapse setup

 

Illumination

Visichrome holographic monochromator

 

Microscope

·       Hamamatsu Orca ER coded CCD Camera

·       Thermostatized incubation chamber

·       Motorized focus

 

Installed filter cubes

·       1: Ex. BP 480/40, Dic. 505, Em.BP 527/30 nm

·       2: Ex. BP 450-490, Dic. 510, Em. LP 515 nm

·       3: Ex. BP 515-560, Dic. 580, Em. LP 590nm

·       4: Ex Triple Band BP 340-380, BP480/40, BP 515-560, Dic. 400, Em. BP 425/?? nm, BP 527/30 nm, LP 590nm

 

Objectives

·       10x

·       20x

·       40x oil

·       63x oil

·       100x oil

 

Attached software:

Metamorph v7, MetaFluor

 

Zeiss Axiovision

 

Illumination

Standard HBO arc lamp

 

Installed filter cubes

·       DAPI BP

·       DAPI LP

·       FITC

·       DsRED BP

·       Rhodamine

·       Alexa 660

·       Polarizer

 

Objectives

·       10x

·       20x

·       40x

·       40x oil

·       63x oil

·       100x oil

 

Attached software:

Axiovision v4.8

 

Nikon Stereomicroscope SMZ 25

 

Motorized stage, filter wheel, focus and zoom.

 

Illumination

Fluorescence,Brightfield and Polarized Light

 

Magnification

·       0.63x up to 15.75x (That's a 25:1 zoom factor)

·       0.5x, 1x and 2x objectives available

 

Filters

DAPI, GFP, CY3

 

Acquisition modes:

Single, MultiChannel, Time-Lapse, Video, Multi Position, Tile Scan, Extended Depth of Focus...

 

Camera

16.25 Megapixels Large CMOS Sensor Color Camera for ultra-high definition images

 

Stereomicroscope Leica MZ16FA

 

*** UPDATE 2015 ***

This setup is currently accessible without booking, to check your samples freely. It has been stripped off of his computer and camera, so no image acquisition is possible. If you need to acquire images, please use the Nikon SMZ25 setup instead. 

 

Illumination

Standard + Fluorescence

Fluorescence Leica EL6000 (lasts up to 2000 hours)

 

Magnification

1x up to 18x

 

Filters

DAPI, GFP, CY3, CFP and YFP on demand (available but not currently installed), Transmitted light

 

Motorized filter wheel, focus and zoom.

 

WORKSTATIONS

 

 

 

Last Updated ( Wednesday, 11 May 2016 )
 
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