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Written by Yannick KREMPP   
Friday, 13 July 2007

MICROSCOPES

 

 

 

 

Leica DMi8 Inverted Fluorescence Microscope

SYSTEM SPECIFICATIONS:

  • Fully motorized inverted microscope
  • Brightfield and fluorescence applications
  • Motorized stage for mosaics and tilescan acquisitions
  • Dry Objectives: 10x, 20x, 40x
  • Oil Objectives: 40x, 63x
  • Filters: DAPI, CFP, GFP, YFP, CY3, CY5
  • Brightfield contrast: Phase on the 10x and 20x
  • Color Camera: Leica DFC7000T
  • Black and White Camera: Andor Zyla
bugnon_leica_dmi8_web.png
 

 

Zeiss Axioscan Z1

SYSTEM SPECIFICATIONS:

  • fully automated for high throughput
  • Z-drive with 3mm usable focus range
  • Magazine for 100 slides 26x76mm
  • Precision scanning stage 300x100mm
  • light source for bright field: Vis-LED
  • light source for fluorescence: HXP 120 V (D)
  • for fixed samples
  • Software: Zen 2

 

 

 

 

 

2 CAMERAS:

Model: 3CCD Hitachi HV-F202SCL for Axio Scan.Z1 (O)

Type: High-End 3CCD progressive scan color camera.

  • Effective number of pixels: 1600 x 1200
  • Cell size: 4.4 µm x 4.4 µm
  • Effective Area: 7.04 mm x 5.28 mm
  • Max frame rate: 30 frames/s RGB 
  • Dynamic range: 12 bit
  • Sensitivity: 2000 Lux/F5.6

 

 

 

 

 Model: Orca Flash 4.0 V2 for Axio Scan.Z1 (O)

Type: High-End monochrome with digital interface

  • Effective number of pixels: 2048 x 2048
  • Cell size: 6.5 µm x 6.5 µm
  • Effective Area: 13.31 mm x 13.31 mm
  • Spectral range: appr. 250 - 1000 nm
  • Camera Link frame grabber 
  • full frame rate: 100 fps, at 20 ms, 
  • Dynamic range: 16 bit
  • Quantum efficiency (at 600 nm): > 70%
  • noise at min EM gain: 1.6 electrons rms

 

img_7814.png
 
 
 
img_7811.png
 

OBJECTIVES

 

 

 

 

Objective

Magnification / NA

Medium

Contrast

Working Distance

Fluar 

5x / 0.25

air

-

12.5 mm

Plan-Apochromat

10x / 0.45

air

-

2.1 mm

Plan-Apochromat

20x / 0.8

air

-

0.55 mm

Plan-Apochromat

40x / 0.95

air

-

0.25 mm

 

FILTERSETS

 

 

 

 

 

Fluorochromes

Availability

Excitation

Dichroic

Emission

Filter set

DAPI

YES

387/11

409

447/602

F36-513

eGFP

YES

470/40

495

520 / 50

38 HE eGFP shift free (E)

Cy3

YES

550/25

570

605/70

43 HE Cy 3 shift free (E)

mPlum

YES

587/25

605

647/70

64 HE mPlum shift free (E)

Cy5

YES

640/30

660

690/50

50 Cy 5 shift free (E)

 

 

 

Zeiss LSM 710 Quasar Confocal Microscope

Illumination
X-Cite lamp

Laser illumination
Diode 405 nm, Argon 458-476-488-514 nm, DPSS 561 nm, He/Ne 633 nm.

Objectives
- Plan neofluar 10x / 0.30 NA (No DIC)
- Plan neofluar 20x / 0.50 NA
- Plan neofluar 40x dry
- Plan neofluor 25x oil immersion
- Plan neofluar 40x / 1.30 NA / oil immersion
- Plan apochromat 63x /1.20 NA / oil immersion
- Plan neofluar 100x /1.30 NA / oil immersion
Equipped with DIC, emission spectra imaging with the Quasar Detector.

Attached software : Zen 2009
lsm-710.png

Zeiss LSM 780 Quasar Confocal Microscope
 
Inverted microscope with fully motorized stand, motorized stage, DIC and Phase, 32 GaAsp detectors, Quasar array for spectral detection and unmixing (34 channels), FCS, Transmission PMT.
 
Illumination
X-Cite lamp
 
Laser illumination
Diode 405 nm, Argon 458-476-488-514 nm, DPSS 561 nm, He/Ne 633 nm.
 
Objectives
- Plan Neofluar 10x / 0.30 NA 
- Plan Apochromat 20x / 0.8 NA
- Plan Neofluar 40x /0.6 NA 
- Plan Apochromat 40x / 1.30 NA / oil immersion
- Plan Apochromat 63x /1.40 NA / oil immersion
 
Software
Zen 2012
lsm-780-small.png

Zeiss Time-Lapse setup

Illumination
Visichrome holographic monochromator

Microscope
- Hamamatsu Orca ER coded CCD Camera
- Thermostatized incubation chamber
- Motorized focus

Installed filter cubes
-1: Ex. BP 480/40, Dic. 505, Em.BP 527/30 nm
-2: Ex. BP 450-490, Dic. 510, Em. LP 515 nm
-3: Ex. BP 515-560, Dic. 580, Em. LP 590nm

-4: Ex Triple Band BP 340-380, BP480/40, BP 515-560, Dic. 400, Em. BP 425/?? nm, BP 527/30 nm, LP 590nm

Objectives

- 10x
- 20x
- 40x oil
- 63x oil
- 100x oil

Attached software : Metamorph v7, MetaFluor

time_lapse_bugnon.png

Zeiss Axiovision

Illumination
Standard HBO arc lamp

Installed filter cubes
-DAPI BP
-DAPI LP
-FITC
-DsRED BP
-Rhodamine
-Alex 660

- Polarizer

Objectives
-10x
-20x
-40x
-40x oil
-63x oil
-100x oil

Attached software : Axiovision v4.8

axiovision_bugnon.png

Nikon Stereomicroscope SMZ 25

Illumination
Fluorescence,Brightfield and Polarized Light

Magnification
0.63x up to 15.75x (That's a 25:1 zoom factor)
1x and 2x Objectives available

Filters
DAPI, GFP, CY3

Motorized Stage

Acquisition modes:
Single, MultiChannel, Time-Lapse, Video, Multi Position, Tile Scan, Extended Depth of Focus...

Camera
16.25 Megapixels Large CMOS Sensor Color Camera for ultra high definition images

Motorized filter wheel, focus and zoom.

nikon-smz25-bugnon.jpg
 



Stereomicroscope Leica MZ16FA

*** UPDATE 2015 *** 

This setup is currently accessible without booking, to check your samples freely. It has been stripped off of his computer and camera, so no image acquisition is possible. If you need to acquire images, please use the Nikon SMZ25 setup instead.  

Illumination
Standard + Fluorescence
Fluorescence Leica EL6000 (lasts up to 2000 hours)

Magnification
1x up to 18x

Filters
DAPI, GFP, CY3, CFP and YFP on demand (available but not currently installed), Transmitted light

Motorized filter wheel, focus and zoom.

 

WORKSTATIONS


2 workstations available

Metafluor / Metamorph

Image acquisition and analysis.

NIH Image / Scion Image / Visilog / Image J
General purpose image analysis software with programming language.

Bitplane IMARIS
Mutli-dimensional image processing, 3D rendering ( shadows, transparencies, perspective projection), animation and stereo projection.


SVI HUYGENS
Deconvolution software

Leica Confocal Software

Zeiss LSM 510 software

Zeiss Axiovision


ROOMS

plan_cif_b.png
click for a larger view

Phone Numbers:

Zeiss LSM 710: 51 65
Zeiss Axiovision: 51 66
Culture Lab: 51 68
Leica SP5: 51 12
CIF Development Unit: 51 09
Corridor: 51 82

MICROSCOPES

 

 

 

Zeiss LSM 710 Quasar Confocal Microscope

Illumination
X-Cite lamp

Laser illumination
Diode 405 nm, Argon 458-476-488-514 nm, DPSS 561 nm, He/Ne 633 nm.

Objectives
- Plan neofluar 10x / 0.30 NA (No DIC)
- Plan neofluar 20x / 0.50 NA
- Plan neofluar 40x dry
- Plan neofluor 25x oil immersion
- Plan neofluar 40x / 1.30 NA / oil immersion
- Plan apochromat 63x /1.20 NA / oil immersion
- Plan neofluar 100x /1.30 NA / oil immersion
Equipped with DIC, emission spectra imaging with the Quasar Detector.

Attached software : Zen 2009
lsm-710.png

Zeiss LSM 780 Quasar Confocal Microscope
 
Inverted microscope with fully motorized stand, motorized stage, DIC and Phase, 32 GaAsp detectors, Quasar array for spectral detection and unmixing (34 channels), FCS, Transmission PMT.
 
Illumination
X-Cite lamp
 
Laser illumination
Diode 405 nm, Argon 458-476-488-514 nm, DPSS 561 nm, He/Ne 633 nm.
 
Objectives
- Plan Neofluar 10x / 0.30 NA 
- Plan Apochromat 20x / 0.8 NA
- Plan Neofluar 40x /0.6 NA 
- Plan Apochromat 40x / 1.30 NA / oil immersion
- Plan Apochromat 63x /1.40 NA / oil immersion
 
Software
Zen 2012
lsm-780-small.png

Zeiss Time-Lapse setup

Illumination
Visichrome holographic monochromator

Microscope
- Hamamatsu Orca ER coded CCD Camera
- Thermostatized incubation chamber
- Motorized focus

Installed filter cubes
-1: Ex. BP 480/40, Dic. 505, Em.BP 527/30 nm
-2: Ex. BP 450-490, Dic. 510, Em. LP 515 nm
-3: Ex. BP 515-560, Dic. 580, Em. LP 590nm

-4: Ex Triple Band BP 340-380, BP480/40, BP 515-560, Dic. 400, Em. BP 425/?? nm, BP 527/30 nm, LP 590nm

Objectives

- 10x
- 20x
- 40x oil
- 63x oil
- 100x oil

Attached software : Metamorph v7, MetaFluor

time_lapse_bugnon.png

Zeiss Axiovision

Illumination
Standard HBO arc lamp

Installed filter cubes
-DAPI BP
-DAPI LP
-FITC
-DsRED BP
-Rhodamine
-Alex 660

- Polarizer

Objectives
-10x
-20x
-40x
-40x oil
-63x oil
-100x oil

Attached software : Axiovision v4.8

axiovision_bugnon.png

Nikon Stereomicroscope SMZ 25

Illumination
Fluorescence,Brightfield and Polarized Light

Magnification
0.63x up to 15.75x (That's a 25:1 zoom factor)
1x and 2x Objectives available

Filters
DAPI, GFP, CY3

Motorized Stage

Acquisition modes:
Single, MultiChannel, Time-Lapse, Video, Multi Position, Tile Scan, Extended Depth of Focus...

Camera
16.25 Megapixels Large CMOS Sensor Color Camera for ultra high definition images

Motorized filter wheel, focus and zoom.

nikon-smz25-bugnon.jpg
 



Stereomicroscope Leica MZ16FA

*** UPDATE 2015 *** 

This setup is currently accessible without booking, to check your samples freely. It has been stripped off of his computer and camera, so no image acquisition is possible. If you need to acquire images, please use the Nikon SMZ25 setup instead.  

Illumination
Standard + Fluorescence
Fluorescence Leica EL6000 (lasts up to 2000 hours)

Magnification
1x up to 18x

Filters
DAPI, GFP, CY3, CFP and YFP on demand (available but not currently installed), Transmitted light

Motorized filter wheel, focus and zoom.

 

WORKSTATIONS


2 workstations available

Metafluor / Metamorph

Image acquisition and analysis.

NIH Image / Scion Image / Visilog / Image J
General purpose image analysis software with programming language.

Bitplane IMARIS
Mutli-dimensional image processing, 3D rendering ( shadows, transparencies, perspective projection), animation and stereo projection.


SVI HUYGENS
Deconvolution software

Leica Confocal Software

Zeiss LSM 510 software

Zeiss Axiovision


ROOMS

plan_cif_b.png
click for a larger view

Phone Numbers:

Zeiss LSM 710: 51 65
Zeiss Axiovision: 51 66
Culture Lab: 51 68
Leica SP5: 51 12
CIF Development Unit: 51 09
Corridor: 51 82

Last Updated ( Thursday, 22 December 2016 )
 
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