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Written by Yannick KREMPP   
Friday, 03 July 2009

epalingeslogo.png MICROSCOPES

INVERTED CONFOCAL MICROSCOPE: ZEISS LSM 880 With Airyscan

Description and info :

The Zeiss LSM 880 confocal microscope

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INVERTED CONFOCAL MICROSCOPE: ZEISS LSM 510 META

Microscope stand configuration

Axio Observer inversé Axio Observer.Z1.
Motorized scanning stage.

Fluorescence filtersets

Filter set 49 DAPI shift free:
EX G 365, BS FT 395, EM BP 445/50.
Filter set 38 Endow GFP shift free:

EX BP 470/40, BS FT 495, EM BP 525/50.
Filter set 43 Cy 3, d=25 shift free:
EX BP 545/25, BS FT 570, EM BP 605/70.
Room for max. 6 cubes for fluorescence on motorized revolver.

Objectives

EC "Plan-Apochromat" 10x/0,45 DIC M27 (WD=5.5mm).
"Plan-Apochromat" 20x/0.8 DIC M27 (WD=0.55mm).

EC "Plan-Neofluar" 40x/1,30 Oil DIC M27 (WD=0.21mm).
"Plan-Apochromat" 63x/1,40 Oil DIC M27 (WD=0.19mm).
"Plan-Apochromat" 100x/1,40 Oil M27 (WD=0.17mm).

Confocal scanner configuration

Available laser lines: 405, 458, 477, 488, 514, 543, 594 and 633 nm.
3 confocal detection channels, including META spectral detector.
1 Transmitted light channel.


Stage-top incubator (for time-lapse)
 
Incubator S with support P for Lab-Tek Chamber.
CTI Controller 3700.
Temp Control 37-2 (2 channels, with objective temperature control and Temp Control mini).


Software

LSM or ZEN 2007.

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UPRIGHT CONFOCAL MICROSCOPE LEICA SP5 TANDEM

Microscope stand configuration

DM5000 microscope
Galvo stage for fast focusing
Motorized scanning stage

Fluorescence filtersets

Filter system A, DAPI:

Ex. filter: BP 340-380, Dichromatic mirror: 400, Suppression filter: LP 425.

Filter system L5,  FITC:

Ex. filter: BP 480/40, Dichromatic mirror: 505, Suppression filter: BP 527/30.

Filter system N2.1, red:

Ex. filter: BP 515-560, Dichromatic mirror: 580, Suppression filter: LP 590.

Room for max. 5 cubes for fluorescence on motorized revolver.

Objectives

HCX PL FLUOTAR 10x/0.30.

HC PL APO 20x/0.70 imm corr.

HCX PL APO CS 40x/ 1.25-0.75 Oil.

HCX PL APO 63x/1.40-0.60 Oil.

HCX PL APO 100x/1.40-0.70 Oil CS.


Confocal scanner configuration

Available laser lines: 405, 458, 477, 488, 514, 543, and 633 nm.
AOBS.
Resonant scanner (25 full frames/s).
3 confocal detection channels (spectral).

Software

LAS AF.


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ArcturusXT Laser Capture and Microdissection

Based on Nikon Eclipse Ti-E microscope.
IR-Laser laser Capture.
UV Laser for cutting.
Objective lenses: 2X, 10X, 20X, 40X.
Phase contrast and Differential Interference Contrast.
Epifluorescence Illumination (Filter sets for  DAPI, FITC, and Rhodamine).
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Stereomicroscope for fluorescence Leica M205FA

General

Motorized Stereomicroscope (focus, zoom, filters, white light).
Remote control with LCD display.
Manual shifting stage.

Illumination

Transmitted light base RCI, 35W.
Incident LED illumination.
Fluorescence light source with fiber optics.

Fluorescence filter sets

Filter Set GFP2 Excitation 480/40.
Filter Set TXR.
Filter SET UV.

Room for max. 4 filter sets on motorized revolver.

Objective

Plan-Apochromat  1X.

Camera, Computer  and Software


Camera Leica DFC300FX R2 (cooled color CCD).
Software LAS AF6000.
Workstation with two monitors 19''.

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Time-Lapse - INVERTED MICROSCOPE  Axio Observer.Z1 motorized
 

Fluorescence filter sets

 
Filter set 49 DAPI shift free:
EX G 365, BS FT 395, EM BP 445/50.
Filter set 38 Endow GFP shift free:

EX BP 470/40, BS FT 495, EM BP 525/50.
Filter set 20 Rhodamin shift free:

EX BP 546/12, BS FT 560, EM BP 575-640.
Filter set 50 Cy 5 shift free:

EX BP 640/30, BS FT 660, EM BP 690/50.
Filter set mCherry (Zeiss):
EX BP 587/25, BS FT 605, EM BP 647/70.

Room for max. 6 cubes on motorized revolver.

 
Objectives
 

EC "Plan-Neofluar" 10x/0,30 Ph1 M27 (WD=5,5mm).
EC "Plan-Neofluar" 20x/0,50 Ph2 M27 (WD=2,0mm).
EC "Plan-Neofluar" 40x/1,30 Oil M27 (WD=0,21mm).
EC "Plan-Neofluar" 63x/1.25 Oil M27.
EC "Plan-Neofluar" 100x/1,30 Oil M27 (WD=0,20mm).


Room for max. 6 objectives on motorized revolver.
 

Stage-top incubator
 

Incubator M with support  for Falcon multiplates (12 wells) or 35 mm dishes (as well as multi-slides). CTI Controller 3700.
Temp Control 37-2 (2 channels) with objective temperature controland Temp Control mini.

 

Camera, Computer  and Software
 

Camera CoolSNAPHQ2.
AxioVision Rel. 4.7 with Module Multi-channel, Z stacks, time lapse.
Workstation QuadCore XEON 2,66 GHz, 22" monitor.

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Zeiss Axiovision

Illumination
Standard HBO arc lamp

Installed filter cubes
-DAPI BP
-DAPI LP
-FITC
-DsRED BP
-Rhodamine
-Alex 660

- Polarizer

Objectives
-10x
-20x
-40x
-40x oil
-63x oil
-100x oil

Attached software : Axiovision v4.8

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Xenogen IVIS Lumina II

The system equipped with an ultra-sensitive camera, a heated stage and gas anesthesia connections for mouse anesthesia (isoflurane). It is designed for bioluminescence and fluorescence measurement in anesthetized animal.

An adjustable field of view from 5 to 24 cm allows imaging of up to 5 mice at the same time. It can also accommodate Petri dishes or micro-titer plates for in vitro imaging.

 

The Xenogen system is located in the C Building, level C01, room 116.

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UPRIGHT MICROSCOPE Axio Imager.Z1 motorized
 

Fluorescence filter sets
 

Filter set 49 DAPI shift free:
EX G 365, BS FT 395, EM BP 445/50.
Filter set 20 Rhodamin shift free:

EX BP 546/12, BS FT 560, EM BP 575-640.
Filter set 38 Endow GFP shift free:
EX BP 470/40, BS FT 495, EM BP 525/50.
Filter set 50 Cy 5 shift free:
EX BP 640/30, BS FT 660, EM BP 690/50.

Filter set CFP (Chroma):
EX BP  436/20, T 455 LP, EM BP 480/40.
Filter set YFP (Chroma):
EX BP 500/20, T 515 LP, EM BP 535/30.


Room for max. 10 cubes on motorized revolver.

 
Objectives

 
EC "Plan-Neofluar" 10x/0,3 M27 (WD=5,2mm).

EC "Plan-Neofluar" 20x/0,50 M27 (WD=2,0mm).

EC "Plan-Neofluar" 40x/1,30 Oil M27 (WD=0,21mm).

"Plan-Apochromat" 63x/1,40 Oil M27 (WD=0,19mm).   

EC "Plan-Neofluar" 100x/1,30 Oil M27 (WD=0,20mm).

Room for max. 6 objectives on motorized revolver.
 

Camera, Computer  et Software
 

Camera AxioCam MRC 5 (color).

Camera AxioCam MRm (black and white).

AxioVision Rel. 4.7 with  Module Multi-channel, Z stacks, time lapse, mark and find.
Workstation QuadCore XEON 2,66 GHz, 22" monitor.

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SkyScan1076 in vivo micro-CT

High performance in vivo X-ray micro-Computed Tomography scanner for preclinical research for producing computer-processed 3-D images specimens.

The SkyScan 1076 scanner is designed for mouse, rat or ex-vivo imaging, at pixel sizes of 9, 18 and 35 µm. Variable X-ray voltage and filters provide scanning flexibility for imaging a wide range of samples ranging from lung tissue to bone with titanium implants. A physiological monitoring system provides breathing and heart-beat gating for thoracic image improvement by synchronized acquisition.

The Micro CT Scan is located in the D Building, 3rd floor, room D325

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LaVisionBiotec Ultramicroscope

(Light Sheet Microscope)

A few technical facts about the system currently installed: 

  • The UltraMicroscope II has a bidirectional triple light sheet technology.
  • This technology allows to generate 6 focused light sheets to excite samples from the side while the fluorescence light is detected by a sCMOS camera perpendicular to the illumination plane.
  • The dynamic horizontal light sheet focus guarantees excellent Z-resolution covering the entire field of view.
  • Moving the sample through the light sheet generates a 3D image stack. Selective excitation of the focal plane reduces bleaching and photo toxicity significantly.
  • The open setup allows the analysis of cleared samples in any clearing solution (organic solvent or aqueous buffer) or in vivo data acquisition in aqueous media.
  • The sample size can vary from mm3 up to 1 cm3. Different working distances also contribute to better image large samples into the depth of the tissue.
  • Four lasers light sources (405 nm, 488 nm, 561 nm and/or a 640 nm laser) are available to adapt to your own applications.
  • The combination of a 2x objective together with a zoom allows variable magnification from to 1.26 to 12.6x.
  • Higher magnification objectives will be added soon.

 

If you plan to use this system, or just have some questions relative to the science behind it, please ask Florence Morgenthaler at the CIF Epalinges.

You can also find more information about that technology right here:

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epalingeslogo.png WORKSTATIONS

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Stations short description


Dell Precision T7400.

2x Intel Xeon 3.20 GHz (1600MHz FSB / 6 MB L2 cache), processor Hyper-threading.
4.0 GB (4x1024MB DIMM) 800MHz DDR2 ECC SDRAM.
512 Mo PCIe x16 (DVI/VGA) nVidia Quadro FX3700.
24" LCD TFT flat wide aspect screen.

Installed softwares

Metamorph
Image acquisition and analysis.

Image J
General purpose image analysis software with programming language.

Bitplane IMARIS suite 6.3.1
Consisting of Imaris, ImarisMeasurement Pro, ImarisTrack and ImarisColoc.
Large range of specific image file readers for 3D microscopes. SLicer, Section, Gallery,
Easy 3D shadow Projection, real time volume rendering.
Interactive 3D distance measurement, volume and voxel intensity statistics in 3D and 4D,
manual object segmentation with contours. Leading 3D and 4D colocalization.

Bitplane ImarisXT - programing interface
Bitplane Filament Tracer 4.1, to segment and visualize filamentous structures
AutoAligner 2.0, for automatic and manual registration of serial 2D images

Leica Confocal Software (LAS AF).

Zeiss LSM 510 software
Zeiss Axiovision

Xenogen IVIS Lumina Software: Living Image 3.0.

epalingeslogo.pngROOMS

All Zeiss microscopes (confocal, time-lapse and upright microscopes) are located in room F212.

Arcturus XT Laser Capture and Microdissection, Stereomicroscope for fluorescence Leica M205FA and Leica SP5 Tandem upright confocal microscope are located in room F213.

Florence Morgenthaler's office and the workstations are located in room F216.

The Xenogen IVIS Lumina II is located in the C Building, level C01, room 116

The Micro CT Scan is located in the D Building, 3rd floor, room D325

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Last Updated ( Monday, 30 October 2017 )
 
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