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CIF Workshops - Deconvolution > Slides PDF
Written by Yannick KREMPP   
Friday, 20 September 2019

Here are the slides from the workshop on Deconvolution (Arnaud Paradis)

 

deconvolution_workshop_slides.png

 

 > download the Deconvolution PDF presentation

 

 


 

 

For those looking for the slides on how to use the HRM server (Yannick Krempp) , find here a link to download the presentation from the workshop.

 
 
hrm_workshop_slides.png

 

 

> download the HRM PDF presentation

 

Feel free to contact the authors if you see errors or have questions.

The CIF staff.

Update 20.09.2019, 20:56 : fixed bad link

Last Updated ( Friday, 20 September 2019 )
 
CIF Workshops - Deconvolution > New Location PDF
Written by Yannick KREMPP   
Tuesday, 17 September 2019

We remind you that the next CIF workshop will be held next Friday at noon on the topic :

"Deconvolution :  Principles [Arnaud Paradis]"

and

"Live Demonstration using Huygens Remote Manager [Yannick Krempp]".

hrm_example_2.png

 

Please take note of the change in location: The workshop will be held at the Bugnon campus, DNF building, Salle APP (aka room 208), entrance level.

 cif_workshops_2019-new-location.png

 

  Registration is free and open to any member of the academic community. Due to room capacity, please register at This e-mail address is being protected from spam bots, you need JavaScript enabled to view it '; document.write( '' ); document.write( addy_text75966 ); document.write( '<\/a>' ); //-->\n This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

 
Image J workshop 2019 material PDF
Written by Yannick KREMPP   
Monday, 17 June 2019
imagej2019_talk.png
imagej2019_demo_outline.png

 

As requested by the workshop attendees, here are the PDFs of the talk and the demo outline for those who want to reproduce some of the steps I have demoed onstage.

 

 

Here's also some of the macros used to process the demo sample (save them as a *.ijm file to use them in FIJI):

Converting a large image to a stack of smaller tiles :

macro "create stack of tiles [s]" { // run("RGB Color"); //ask for user input n = getNumber("How many divisions?", 2); id = getImageID(); title = getTitle(); getLocationAndSize(locX, locY, sizeW, sizeH); width = getWidth(); height = getHeight(); tileWidth = width / n; tileHeight = height / n; newImage("TilesStack", "RGB composite-mode", tileWidth, tileHeight, 1, n*n, 1); idStack = getImageID(); for (y = 0; y < n; y++) { offsetY = y * height / n; for (x = 0; x < n; x++) { offsetX = x * width / n; selectImage(id); call("ij.gui.ImageWindow.setNextLocation", locX + offsetX, locY + offsetY); tileTitle = "title="+ title + " [" + x + "," + y + "]"; makeRectangle(offsetX, offsetY, tileWidth, tileHeight); run("Copy"); selectImage(idStack); run("Paste"); run("Next Slice [>]"); } } selectImage(id); close(); }

Processing a tile :

macro "Filter image and Analyze [f]" { // Make sure you don't touch the original image - useful for batch processing run("Duplicate...", "Copy of original image"); run("RGB Color"); // Ask for user input medianRadius = getNumber("Type in the median radius: ", 2); // Do the segmentation run("Median...", "radius=" + medianRadius); run("Color Threshold..."); // Color Thresholder 2.0.0-rc-68/1.52h // Autogenerated macro, single images only! min = newArray(3); max = newArray(3); filter = newArray(3); a = getTitle(); run("HSB Stack"); run("Convert Stack to Images"); selectWindow("Hue"); rename("0"); selectWindow("Saturation"); rename("1"); selectWindow("Brightness"); rename("2"); min[0] = 145; max[0] = 184; filter[0] = "pass"; min[1] = 21; max[1] = 255; filter[1] = "pass"; min[2] = 170; max[2] = 255; filter[2] = "pass"; for (i = 0; i < 3; i++) { selectWindow("" + i); setThreshold(min[i], max[i]); run("Convert to Mask"); if (filter[i] == "stop") run("Invert"); } imageCalculator("AND create", "0", "1"); imageCalculator("AND create", "Result of 0", "2"); for (i = 0; i < 3; i++) { selectWindow("" + i); close(); } selectWindow("Result of 0"); close(); selectWindow("Result of Result of 0"); rename(a); // Colour Thresholding------------- //setThreshold(255, 255); setOption("BlackBackground", true); run("Make Binary"); // Clean the segmentation run("Median...", "radius=2"); run("Fill Holes"); run("Watershed"); // Specify what has to be measured and analyze run("Set Measurements...", "area perimeter redirect=None decimal=3"); run("Analyze Particles...", "size=50-Infinity display exclude clear add in_situ"); }

 

Yannick Krempp

Last Updated ( Monday, 17 June 2019 )
 
Workshops 2019 PDF
Written by Yannick KREMPP   
Friday, 10 May 2019

The schedule of the next series of workshops given by the CIF is now live :

cif_workshops_2019-new-location.png

These workshops are intended for max 15 pers. Registration (free) is recommended (mail to Jean-Yves Chatton ).

The students of the doctoral schools (neuroscience, FBM, etc.) can validate their participation to a workshop as one seminar for their credits.
  
Links to previous workshops content and documents:
http://cifweb.unil.ch/index.php?option=com_docman&task=cat_view&gid=21&Itemid=57

 

Last Updated ( Tuesday, 17 September 2019 )
 
Welcome to our new collaborator, Luigi Bozzo ! PDF
Written by Yannick KREMPP   
Friday, 11 January 2019
This CIF platform is happy to welcome a new collaborator, Luigi Bozzo.
 
 
lbozzo_photo_1.jpg


Here's a short bio about him:
 
Luigi Bozzo has a solid theoretical and practical knowledge in light microscopy accumulated over 10 years of experience. He started his career doing a PhD in neuroscience at UNIL on the functional interplay between neurons and glial cells. To gain access to spatial and temporal aspects of these dynamic interactions, he used optical microscopy, associated with specific fluorescent indicators on living tissues. 
 
Then he entered the world of core microscopy facilities as an ad interim technical manager at the CIF before being hired as microscopist at the Bioimaging and Optics Platform (BIOP), EPFL. He was dealing with all aspects of modern light microscopy in particular in the field of life sciences and was involved in all aspects of the core microscopy facility. He was exposed to different microscopy techniques like wide-field imaging, confocal imaging up to super resolution methods. In particular, he gained expertise in advanced techniques such as fluorescence life-time imaging microscopy (FLIM), Fluorescence Resonance Energy Transfer (FRET), Fluorescence recovery after photobleaching (FRAP), spectral unmixing, and intelligent image acquisition (fully automated acquisition workflow).

As technical manager/microscopist, he came in contact with scientists from very diverse backgrounds, from physics to medicine. He helped them by supporting their scientific projects; advising them on experimental designs; discussing staining strategies; choosing the appropriate microscope; providing assistance to obtain the optimal image acquisition and analysing the output. He successfully trained more than 400 users to the usage of microscopy systems.

Luigi Bozzo started on Dec. 1st 2018 as technical manager of the CIF AGORA/BUGNON facility branch. He will assist on the trainings on the Bugnon Campus as well as taking care of the new Agora CIF facility. He will also participate in every other activities of the CIF platform in general.
 
You can reach Luigi at: 

  • Office phone + 41 21 545 10 60 / + 41 79 556 95 52
  • Office address: Centre de recherche AGORA – Cellular Imaging Facility – Rue du Bugnon 25A – Bureau 248 CH 1011 Lausanne.
  • Email : This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

Last Updated ( Friday, 11 January 2019 )
 
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