Development Platform
Written by Yannick KREMPP   
Thursday, 28 June 2007

TWO PHOTON FOR LIVE IMAGING ON BRAIN SLICES DEVELOPPED AT CIF

 

The CIF development unit is currently designing and building a 2-photon microscope for live-imaging in thick tissues. It is first built with experiments on brain slices in mind, but could accommodate other experimental situations as well.

 

Two-photon microscopy uses an infrared pulsed (“femtosecond”) laser to obtain epifluorescence images from biological samples. This feature allows for deeper imaging in tissue than when using a visible or UV lasers like in confocal microscopy. Moreover, the 2-photon absorption phenomenon means that only the focal plane will emit the fluorescent signal thus permitting optical sectioning without a pinhole. The result is an increased sensitivity of detection making this technique useful for low-light level imaging in living samples.

 

The current development was triggered by the need of having more affordable, more flexible and upgradeable instruments for imaging on live samples than what is usually provided on commercial apparatus. Typically, commercial 2-photon microscopes are high-end expensive instruments that are hard to integrate to an experimental environment where multiple parameters, like electrophysiology or stimulations, need to be monitored at the same time as the optical signal.

 

Future projects based our microscope design will focus for instance on implementing in-vivo 2-photon imaging and fast 3D scanning, testing cheaper and more compact laser sources and the development of new imaging modalities like second-harmonic generation. 

 cif_rd_platform_web.png

 Poster available as PDF: view poster  

ADVANTAGES OF OUR MICROSCOPE

Affordable and accessible
Two-photon technology made accessible to FBM researchers at a lower cost

Flexible and user driven
New functionalities needed by users for specific experiments can be implemented

Full of features
Easy to run complex experiments integrating imaging, electrophysiology and stimulus conditions

Collaborative
Software is open source, design is available for duplication

High tech
Microscope will benefit from latest technological innovations

  Please fill this survey : CIF 2P Survey

Last Updated ( Tuesday, 08 February 2011 )